Genetic preservation begins with the painless collection of tissue
by a veterinarian. While under local or general anesthesia your
veterinarian will use a biopsy punch or other surgical instrument
to obtain two pieces of skin each approximately the size of a pencil
eraser. These tissue samples are then shipped in an insulated container
with an ice gel pack by overnight courier to the PERPETUATE's
laboratory in Massachusetts where they are cultured.
Culturing involves first segregating desired cells; placing the
desired cells in an optimal controlled growth environment; and continuing
to grow the cells until a cell line of a million or more cells is
achieved. On average it takes two to four weeks to culture a cell
Cell Line Cryopreservation
Once a cell line with your pet or horse's DNA is established, the
cell line is cryopreserved by a graduated freezing process to extreme
temperatures (-320° F.) Cryopreservation is a unique biotechnology
that permits living cells to be preserved at extremely low temperatures
then thawed and used for cloning or other purposes.
Cell Line Storage
Cryopreserved vials of cells are placed in liquid nitrogen in PERPETUATE's
maintains two separate storage units one of which owners can visit.
The location of the second Bio-Kennel is not disclosed for security
reasons. Cells can be kept viable in liquid nitrogen for decades.
Genotypes or 'DNA signatures' are developed for each animal that
cultures and cryopreserves. The genotypes are produced by the independent
Veterinary Genetics Laboratory at the University of California in
Davis, California and are used to identify the animal, cell line
Certificate of DNA Preservation
(Click photo for larger rendering)
To enter your special pet or horse in our program,
- or -