PERPETUATE is pleased to report that four clones of Charlot will be delivered to their owners in Florida on June 1st. The clones were born in April 2017. Charlot's cells were cryopreserved by PerPETuate in January 2000. Charlot was a male Papillion.


PET CLONING OBSERVATIONS #1

PerPETuate cultures and cryopreserves genetics from exceptional pets! Owners consistently state that their cat or dog was the most incredible pet that they ever owned. Superlatives like brilliant, extraordinary, gifted, incomparable, talented and smart are repeatedly used to describe these unique animals.

PerPETuate believes the common denominator amongst these remarkable animals is intelligence. Their extraordinary intelligence enables them to learn easily and quickly, understand and establish exceptional bonds with their owners.

After five years of observing a limited number of cloned pets, PerPETuate is able and willing to make these preliminary observations: - Cloned puppies and kittens from exceptional donors appear to be more intelligent than normal progeny. - Cloned puppies and kittens from exceptional donors are incredibly happy, active and responsive to commands. PerPETuate expects these observations to be scientifically confirmed in the future.


CRYOPRESERVING CELLS

Cryopreservation is the final step in PerPETuate's cell culturing process. Cryopreservation begins when cultured cells are transferred from Petri dishes and to small vials of cryoprotectant medium. After labeling, the vials are cooled slowly by a programmable rate freezer to -112°F. The vials are then placed in liquid nitrogen refrigerators at temperatures below -300°F. Cryopreserved cells can be maintained in liquid nitrogen for decades.

PerPETuate stores our clients' cells in two separate storage facilities. Pet owners can visit one of the facilities. The second store's location is not disclosed for security reasons.

The following video explains cell cryopreservation in more detail.


DOLLY’S 20TH ANNIVERSARY

February 22nd. was the 20th anniversary of Dolly’s cloning announcement. Dolly the sheep was born on July 5, 1996 in Scotland. She was cloned at the Roslin Institute by Drs. Keith Campbell and Ian Wilmuth.

Dolly had three mothers (one provided the egg, another the DNA and a third carried the cloned embryo to term). She was created using the technique of somatic cell nuclear transfer. She was the first clone to be produced from an adult mammal cell.

Dolly lived her entire life at the Roslin Institute in Edinburgh. There she was bred with a Welsh Mountain ram and produced six lambs in three pregnancies.

Dolly died from lung cancer on February 14, 2003. Any connection between Dolly being a clone and her early death were dismissed. First other sheep in Dolly’s flock died of the same disease as she did. Second four other Dolly clones were produced after Dolly all of which outlived her and are still living.

Read more at the Economist


WOOLLY MAMMOTH CLONING UPDATE

PerPETuate clients with stored non-viable tissue will be interested in a new approach to cloning the woolly mammoth. Harvard scientists are trying to rebuild the mammoth genome by replacing some elephant genes with mammoth ones.

The new approach follows South Korean scientists' failure to recover significant amounts of woolly mammoth DNA in Siberia. Most of the uncovered mammoth tissue's DNA was destroyed after being frozen for centuries. ... See More

Woolly mammoth on verge of resurrection, scientists reveal Scientist leading 'de-extinction' effort says Harvard team could create hybrid mammoth-elephant embryo in two years


Pet Owner Question: "Our beloved old cat just started chemo after being diagnosed with large cell intestinal lymphoma. We would love to preserve his DNA but I am wondering if the chemo and/or the cancer would affect the results? Have you worked with others in this situation? Thanks!"

PerPETuate Answer: "We have successfully cultured cells from hundreds of pets that were suffering with cancer and were undergoing chemo or radiation treatment." Feb 4, 2017


CLONING TISSUE REQUIREMENTS

Pet owners frequently ask PerPETuate if fur, teeth or nails can be used to clone their pets. The answer is 'no.' Even when fur, teeth or nail tissue is viable, their cultured cells cannot be used for cloning.

PerPETuate's practice is to use skin tissue from live and muscle tissue from deceased animals to produce cell lines. Adipose tissue is also acceptable. Blood, treated with heparin to prevent coagulation, has in a very limited number of cases produced viable pet cell lines. Cells cultured from these four types of tissue can be used to clone pets.


Happy Holidays!

PERPETUATE wishes our clients, friends and their pets HAPPY HOLIDAYS!


PERPETUATE'S FIRST FELINE CLONES

PerPETuate's first feline clones are more than one year old. Both clones were produced from a PerPETuate cell line at ViaGen's cloning laboratory on September 27, 2015. The European owner, who chooses to remain unidentified and not to publicize images of his original cat, is completely satisfied with the clones. Both clones are incredibly similar physically and behaviorally to the original cat and to one another.


BOB SEMEL'S CLONES

Bob Semel, a spirited Deer Chihuahua, lost his life in September 2011. Clones of Bob, produced from cells cryopreserved by PerPETuate, were born in January 2012. Bob's clones will celebrate their 5th birthday next January.


CHAUSIES

Morpheus was the first Chausie that PerPETuate cell banked in 2002. Chausie is a domestic breed of cat that was developed by breeding domestic cats with a few species of nondomestic jungle cats. Morpheus was tall, lean, with very long legs, deep-chested, tall ears and striking eyes like other Chausies.


CLONED ANIMALS ARE HEALTHY AND SAFE

Twenty years after the birth of the world’s first clone—Dolly the Sheep—extensive research has proven that cloned animals are healthy, safe and age normally. These conclusions result from analyzing thirteen clones of Dolly during the last fourteen years. All of the clones including four, who are now eight years old, are free of the diseases that caused Dolly’s premature death when she was six years old.

Animal cloning is increasingly more reliable, better understood and accepted by animal owners and the public. New cloning applications like stem cell therapy, medical and behavioral R&D, preserving endangered species, reviving extinct ones and other unique prospects, bodes well for the future of animal cloning.

For more information, please see: http://www.nature.com/ncomms/2016/160726/ncomms12359/full/ncomms12359.html


“CLONING TAKES GUESS WORK OUT OF BREEDING”

John Brannon, a dog trainer, has three clones of his top police service dog. He trains dogs primarily to search for drugs or explosives and to protect their handlers.
Brannon has successfully completed training of two of his clones and placed them with police SWAT units. Training the third clone—Spector—is going well.

Brannon says cloning seems to take the guess work out of normal breeding procedures. He said even when you breed the best female dogs with top male police dogs no more than 50% can be trained to be police or military service dogs. See following link for more information:

http://www.wfmj.com/story/32075195/clone-police-dog-in-training-in-mercer-county


PET CLONING UPDATE

Dog and cat cloning are on verge of making significant progress in near future. Pet cloning takes two forms—therapeutic and reproductive.

Therapeutic cloning commonly known as stem cell therapy is a burgeoning new industry focused on pet diseases and therapies. Stem cells for example are employed increasingly to treat osteoarthritis and bone, joint and ligament injuries for dogs and stomatitis and other chronic cat diseases.

Reproductive cloning has made less progress than pet therapeutic cloning. No more than twenty cats were cloned after 2003 when the first cat was cloned. The number of dogs cloned since 2005 is estimated to be near 300 all of which were cloned in South Korea. In past year improved cloning biotechnologies and significant American investments in pet cloning are promising news for pet owners.


TESTIMONIAL PerPETuate,

Thanks for the great communications during the process, it was an unexpected plus. There is a sense of security for the family having completed the cell line. When I have discussed with friends and colleagues none thought it 'odd' - or at least said so to me. PR - April 2016


SHANNON DILLER CLONES

Media mogul Barry Diller, the chairman of IAC, and his wife, designer Diane von Furstenberg have cloned their famous Jack Russell terrier--Shannon. They are now the proud owners of cloned pups, Deena and Evita.

Cloning dogs costs up to $100,000 and is done by a Korean firm that implants DNA into a dog egg. A rep for Diller confirmed the cloning.


SCIENTISTS IN IRAN CLONE RARE MOUFLON

A domestic sheep has given birth to a baby mouflon in a rare successful example of interspecies cloning, according to scientists in Iran. The wild Isfahan mouflon - or ovis orientalis isphahanica - was cloned by researchers at Iran's Royan Institute, which is dedicated to reproductive biomedicine and stem cell research, using the domestic sheep as a surrogate mother. Poaching has driven the Isfahan mouflon close to extinction in Iran. The cloned mouflon, which looks similar to a deer, has been named Maral, a Persian name for a reindeer and for new babies, which means svelte. For more information click here.


SCOTTISH SCIENTISTS ANNOUNCE PLANS TO CLONE ENDANGERED GIANT PANDAS

Currently, there are only about 2,000 giant pandas left in the world and in an attempt to save giant pandas from extinction, scientists have announced plans to clone Tian Tian and Yang Guang who currently reside at Edinburgh Zoo in Scotland. The same experts who cloned Dolly the sheep have already taken tissue samples from the giant pandas and produced viable cell lines to genetically preserve them for later cloning use. This cloning project is the first step in making genetic copies of the endangered animals following a failed attempt to artificially inseminate Tian Tian in March. For more information click here.

 


KOREAN CLONES TO ENTER SERVICE AS RESCUE DOGS

Dasol and Nara, two dogs cloned by dog cloning pioneer Hwang Woo-suk and his Sooam biotech team, have been accepted into Korea's rescue dog program becoming what are assumed to be the world's first clones to enter service as rescue dogs. The National 119 Rescue Service of the Ministry of Public Safety and Security announced that the two clones donated by Hwang, Dasol and Nara, have passed the official qualification tests for rescue dogs. Both dogs were cloned from donors whom had previously been enlisted as service dogs and are showing great promise to follow in the same footsteps. Read more here: http://ow.ly/V5y8r


EXTINCT PREHISTORIC CAVE LION UNDER CONSIDERATION FOR CLONING BY RUSSIAN SCIENTISTS

In November Russian scientists discovered the best preserved remains to date of two baby cave lions whom likely died out 12,000 years ago around the same time as the Mammoths. "The remains are unique and know no equals across the world," the local Academy of Sciences' statement reads. "Since the cubs have well-preserved soft tissues, we, the scientists, tend to believe that they can be cloned. But we can speak about the results of this project in two or three years." Scientists from Japan, South Korea and the United States are joining in the study. Read more: http://ow.ly/V5y3L


FIRST CLONED CAMEL GIVES BIRTH IN DUBAI

The first cloned camel gave birth to a healthy happy female calf on Nov. 2nd at the Reproductive Biotechnology Center in Dubai. Several more cloned camels are due to give birth next year. For more information visit http://ow.ly/V5xYv


HAPPY NEW YEAR PERPETUATE CLIENTS AND FRIENDS

 


CHAMPION POLO PONY CLONES

Star polo player Adolfo Cambiaso will be taking at least four clones onto the field of the Palermo Open in an attempt to recapture the success of their polo champion donors. Cambiaso says that 85%of polo clones are as good on the field or better than their original donor horses. For more information on polo pony cloning click: http://www.independent.co.uk/news/world/americas/polo-pony-cloning-is-set-revolutionise-the-sport-at-argentinas-palermo-open-a6715646.html


THIS YEAR GIVE THANKS FOR PETS

Pets are our friends, our confidants and our constant companions. Whether they're devoting their time to their loving families or dedicating their lives to service work our furry companions are constantly giving back. This year join us in giving thanks for all the wonderful pets that have touched our lives and give back to them by giving them the gift of genetic preservation. PerPETuate preserves DNA by culturing and cryopreserving viable cells from exceptional pets and horses. Cryopreserved cells can be used to clone cats, dogs and horses, perpetually preserve their bloodlines and for medical diagnosis and treatment.


PET HOSPICE

Pet hospices provide comfort-oriented care to companion animals as they approach the end of life. The professional pet hospice movement began in the 1990s. Since then the number of pet owners who use and number of veterinarians who offer hospice services have and are increasing. The International Association of Animal Hospice and Palliative Care was formed a few years ago to develop guidelines and to promote research and scholarly discussion animal hospice practices and issues.

The Lap of Love Hospice is the largest pet hospice in the USA. Dr Mary Gardner co-founder of the Lap of Love Hospice discusses animal hospice in the following New York Times article: http://www.nytimes.com/2013/12/01/business/all-dogs-may-go-to-heaven-these-days-some-go-to-hospice.html?_r=0


CLONES OF BOB SEMEL

Bob Semel's clones are now fully grown and nearly four years old!

PerPETuate clients and friends will remember Bob Semel and his three clones. The clones were born in South Korea in January 2012 following Bob's tragic death in August 2011. The three clones-Bob, Jim and Tom-were some of the first dogs to be produced from a PerPETuate cell line.

From the time the clones reached their Los Angeles home in March 2012, Dr. George Semel their owner continued to be amazed with how similar the clones were to Bob and to one another in terms of temperament, personality and physically. One of the three clones-Tom-was lost in a traffic accident in when he was a year old. The other two clones-Jim and Bob-are now fully grown, healthy and full of life. See video of clones in post below.


FIRST ANNUAL DOG FILM FESTIVAL SET FOR THIS WEEK

The First annual dog film festival will take place on October 3, 2015 from 11 a.m. - 11 p.m. at the prestigious Peter Norton Symphony Space, a 765-seat theater in New York City. The festival has a full line up set to delight from doggy swag bags to "dogumentaries" it's poised to be a barking good time.

http://www.dogtipper.com/blog/2015/02/first-annual-dog-film-festival-announced.html


DNA PROFILING

DNA profiling was developed and first used in 1985 to identify individuals by their DNA traits. DNA profiles are comprised of a limited number of specific DNA genes that are unique to an individual like fingerprints. Today DNA testing is used extensively to identify individual persons and for forensic purposes.

In the 1990s the International Society of Animal Genetics began developing standardized DNA profiles for pets and livestock. DNA profiles are known alternatively as DNA signatures or fingerprints, genetic-marker reports, testing and genotypes.

PerPETuate maintains that DNA profiles present a method to certify scientifically animal clones. The donor pet's DNA signature must be identical to the clone's DNA signature or it is not a clone! Since 2000 the University of California-Davis Veterinary Genetic Laboratory has provided PerPETuate clients with DNA signatures of their pets.

DNA profiles use is limited to the identification of the individual animal and its parents, cells and clones. Other types of DNA biotechnology such as sequencing techniques can be used to determine the breed, color, disease, lineage and so forth.


DE-EXTINCTION

De-extinction is the process of creating an organism that is or resembles a member of an extinct species. De-extinction efforts have not yet succeeded in producing viable offspring of a previously extinct species, the process however has been applied successfully to endangered species such as the gaur.

PerPETuate has cryopreserved non-viable cells from many pets that could not be cultured often due to freezing. Our clients are anxiously following the advancement of de-extinction research and development with the hope that will enable them to clone their pets in the future.

View the attached video of de-extinction efforts to revive the extinct passenger pigeon: http://www.nationalgeographic.com/deextinction/


WORLD'S FIRST ANIMAL AIR TERMINAL

New York's J. F. Kennedy Airport can look forward to a new luxury terminal - The ARK- that will handle the more than 70,000 animals flying in and out the USA every year. The ARK will be the world's first air terminal for pets and other animals. Set to open next year the $48 million, 178,000-square-foot shelter and quarantine facility will be able to accept all species and large numbers of pets and animals.

The ARK will offer a 20,000-square-foot "resort" run with a bone-shaped splashing pool for dogs and cats will have their own trees to climb. All animals will have access to a 24-hour clinic operated by Cornell University's veterinary college

More information available at: https://www.youtube.com/watch?v=1Z4ciR02FAk


DOG CLONING CHALLENGES

Dogs are the most difficult of all mammals to clone. Problems begin with fact that the canine reproductive physiology is significantly different from other species. Many procedures used in livestock cloning cannot be applied to dogs.

The limited supply of canine oocytes or immature eggs is another important challenge. Large numbers of oocytes are needed to clone dogs. Canine females tend to ovulate randomly and only twice a year. In addition they produce fewer eggs per ovulation than do other species. And mating does not trigger ovulation.

Another major challenge is that after an oocyte matures it is viable for only a few hours versus several days for other species. The entire canine cloning process from egg collection, evaluation and synchronization to somatic cell nuclear transfer and finally to transferring the cloned embryo to a surrogate mother must be completed within no more than ten hours!


GENETIC ENGINEERING: THE INNATE POTATO

The Innate Potato is a GMO creation that was recently approved for cultivation in the US. Genes from a wild Mexican variety of potato were added to the popular species of Russet Burbank potatoes resulting in the Innate Potato.

The Innate Potato is an example of how genetic engineering is positively benefiting mankind as it:
• Reduces the amount of acrylamide produced by potatoes by as much as 70%. Acrylamide is a known carcinogen at high doses.
• Reduces some of the $300 million annual potato waste caused by bruising. Potato bruising results from enzyme released during harvest and transport. Enzyme release is controlled in the Innate Potato.
• Improves starch quality and potato color.
• Requires no additional pesticides or chemicals.
• Does not sequence cross species.
• Is not linked to Monsanto.

Read More Here


SKIN TISSUE BIOPSIES:

Skin from living pets is the best source of tissue for genetic culturing and preservation. PerPETuate recommends that a biopsy punch with its circular blade be used to collect two 3 to 4 mm full-thickness skin samples (each approximately size of pencil eraser). The procedure begins by administering a local or general anesthesia and then by shaving and sterilizing the biopsy site. Skin punch biopsies usually take less than 30 minutes to complete.

Biopsy Site Selection: Skin samples should not be taken from areas where bacteria tend to concentrate such as feet, rump and mouth. Possible infected skin should be avoided. The scruff of the neck is an advantageous site as it is easily assailable, clean and cannot be licked by the pet after the biopsy.

Contact PerPETuate at 808-989-2028 or rdgille@gmail.com for more information.


SCIENTISTS MAP WOOLLY MAMMOTH GENOME

Scientists have completed mapping the woolly mammoth genome. This genetic road map will be of considerable use in efforts underway to clone the woolly mammoth. It also underscores the value and potential of animal genetic preservation.

Scientists Map Mammoth Genomes, Which Could Help Bring Beasts Back To Life

Scientists may be one step closer to bringing the woolly mammoth back to life. In a new study, an international team of researchers has sequenced the genomes of ... Click here for more


BIOPSY FACTS AND RECOMMENDATIONS

• Genetic preservation requires small portions of viable tissue from your pet.
• Skin is the preferred type and source of tissue from living pets.
• Muscle is the preferred type and source of tissue from deceased pets.
• Tissue must be harvested in a germ-free environment.
• Local or general anesthesia is necessary for skin sample collection.
• Combining skin collection with teeth cleaning or other operation is recommended.
• Tissue needs to be collected as soon as possible from deceased pets.
• It is recommended that tissue be collected immediately after a pet is euthanized.
• Drugs used to euthanize animals do not affect cell culturing.
• Deceased animals must be kept at refrigerated temperatures until tissue is harvested.
• Tissue usually loses its viability within five days of death.
• Call PerPETuate for more information at 808-989-202.

 


ENDANGERED AND EXTINCT SPECIES

PerPETuate's primary goal is to preserve genetics from exceptional pets and horses enabling these genes to be used for cloning or genetic improvement. Additionally PerPETuate encourages the use of genetic preservation and cloning for endangered breeds and species. For example in 2001 PerPETuate was associated with Advanced Cell Technology when they cloned a gaur--an endangered Asian ox.

Since PerPETuate's founding in 1998 the use of genetic preservation, even cloning endangered animal and plant species has achieved worldwide acceptance and practice. Today scientists are pursuing the much more daunting task of reviving extinct species through cloning.

Click here for more.


HAPPY NATIONAL PIG DAY FROM PERPETUATE!

March 1st marks the 43rd annual celebration of National Pig a day, a holiday created to accord the pig its rightful, though generally unrecognized, place as one of man's most intellectual and domesticated animals. This month also serves as the 15th anniversery of the announcement of the very first pig clones produced by a team of PPL Theraputic scientists in Blacksburg, VA and PerPETuate’s very own lab manager, Dr. Ray Page, was part of the team.


Happy Birthday Charles Darwin!

Charles Darwin revolutionized the world with his theories on evolution and inherited traits passed on from parents to offspring in the early 1800's. Now, more than two centuries later, the ideas set forth by the great grandfather of genetics has not only shaped our views on breeding plants and animals but opened our eyes and minds to the infinite possibilities of genetic research. February 12th marked the 206th anniversary of the birth of Charles Darwin and a summary of his impact on genetics through the years can be found here


FELINE GENOME

Since the dog genome was sequenced ten years ago geneticists have identified hundreds of canine diseases and physical trait genes. By comparison only a limited number of such genes have been identified in cats.

In 2007 the first cat genome was sequenced. Errors and gaps however stalled efforts to map genes. A complete, high-resolution feline genome was published in 2014. One if its first discoveries was that cats after 9,000 years of domestication are quite similar to their original feral counterparts!


Geneticist Leslie Lyons of the University of Missouri has launched a feline genetics research project called ‘99 Lives Initiative’ to improve the quality of data and identify genetic variations behind specific feline diseases. Humans stand to benefit from ’99 Lives’ as cats and humans share similar diseases such as type 2 diabetes, asthma, retinal atrophy and HIV.

Click for more


KARMA

PerPETuate recently preserved genetics from Karma, a beautiful female African Lion. Following Karma’s untimely death The Black Jaguar-White Tiger Foundation undertook extraordinary efforts to hand carry and deliver Karma’s blood samples from Mexico City to PerPETuate’s laboratory in Worcester, Massachusetts.

The Black Jaguar-White Tiger Foundation rescues cruelly treated Big Cats from circuses, zoos and illegal breeders. In 2013 the Foundation’s founder rescued a newly born Black Panther cub about to be euthanized after her mother died during birth. Since then the Black Jaguar-White Tiger Foundation has rescued more than 30 lions, tigers and jaguars. You can learn more and support The Black Jaguar-White Tiger Foundation by visiting their website: www.blackjaguarwhitetiger.org


TIBETAN MASTIFF CLONES

Three Tibetan Mastiff clones were produced in China. Tibetan Mastiffs are one of the oldest, rarest and most expensive canine breeds. Tibetan herders have used them as shepherd dogs since 1100 BC. They are solemn and fierce simultaneously kind and loyal. Their immense double coat protects them from sever Himalayan cold weather. They can be black, brown and blue and may have tan or gold markings.

The three puppies were cloned from a dog named Jiama in China’s Qinghai-Tibet Plateau. Eight-year old Jiama has won several Tibetan Mastiff breed championships and her puppies reportedly have been sold for more than $2 million!
See following link for more information: http://sputniknews.com/society/20140925/193268073/China-Starts-Commercially-Cloning-Tibetan-Mastiff-Puppies.html


Happy New Year from PerPETuate

Visit our Facebook page and enjoy a compilation of our fabulous pets from 2014 as they wish you a very Happy 2015!. See Video Here.


Penny PerPETuate here along with my trusty reindog sledding by to wish you and yours Happy Holidays!

 


ANIMAL PARADISE

Pope Francis recently consoled a small child whose dog had died by assuring him that “one day, we will see our animals again” and that “paradise is open to all of God’s creatures” according to widely cited Italian media reports.

For more details: http://www.huffingtonpost.com/.../pope-francis-animals-heaven...


Cyber Monday is almost here!

So don't forget to spread the good cheer! Genetic preservation from PerPETuate, Inc. Is the only gift this holiday season that gives pet owners the piece of mind that their exceptional pet's DNA is preserved indefinitely. Genetic preservation not only offers pet owners a head start on cloning but also access to their exceptional pet's genes that can be used to check for genetic diseases and hereditary traits as tests become available. Order your $100 tissue collection kit today at www.perpetuate.net


DOG AND WOOLLY MAMMOTH CLONING

The Smithsonian Channel features efforts by the Sooam Foundation of South Korea’s involvement in attempts to clone an extinct Woolly Mammoth in a new video. The video offers excellent dog cloning and woolly mammoth visuals as well as raises some of the controversial issues. You can access the link at: http://www.smithsonianchannel.com/sc/web/show/3415041/how-to-clone-a-woolly-mammoth#would-you-pay-100000-to-clone-your-dog


CATS ARE LESS DOMESTICATED THAN DOGS

After 9,000-odd years of living alongside humans, the house cat is only semi-domesticated according to recent DNA studies by scientists at Washington University in St. Louis. Domestic cats still have many of the same traits as their wild cousins. For example the fact that house cats unlike dogs retain their ability to hunt and survive effortlessly in the wild underscores how little impact humans have had on them.

The genomic study on the other hand concluded that the humans have had some influence on cats. For example stem cell changes have resulted in more docile felines and humans have selectively developed cat breeds for preferred coat colors, patterns and fur types.

Refer to the following link for more of the DNA research findings.
http://www.latimes.com/science/space/la-sci-sn-cat-genome-20141107-story.html


Penny PerPETuate here to wish everyone a magical Happy Halloween!

Penny and the entire PerPETuate family hopes you and your exceptional pets have a cackling good time today!


NATIONAL CAT DAY

National Cat Day is October 29. PerPETuate loves to celebrate our feline friends! What better way to celebrate your exceptional kitty than to preserve his or her genes for years to come? PerPETuate Inc. the world’s most experienced genetic preservation company, has been genetically preserving exceptional cats since 1998. For more info on how to take the first steps towards genetically preserving your pet visit www.perpetuate.net


REPTILE GENETIC PRESERVATION AND CLONING

As National Reptile Awareness Day nears, PerPETuate expects more questions regarding whether it is possible to clone turtles, snakes, lizards or other species of reptiles. We believe it is possible to clone reptiles but know of no successful cloning attempt to date.

PerPETuate can develop reptile cell lines and preserve their genetics for future cloning. Preserving reptile cells involves a different procedure than used in preserving dog and cat genetics. For more information contact PerPETuate at 808-989-2028.


PRESERVING CHAMPION GENES FOR FUTURE GENERATIONS

Genetic preservation offers horse owners a unique new opportunity to perpetuate the bloodlines of past champions for future generations. Highly competitive horses are at a greater risk of illness, injury and accidental death due to the rigors of training, traveling to competitions, and the increased risk of exposure to disease at these big events. By genetically preserving these horses owners are insured the bloodlines of these horses remain safe and give owners the opportunity to have their "once in a lifetime horse" twice through cloning. The benefits of genetic preservation aren't just limited to cloning possibilities, they also allow stallion owners the opportunity to test for specific genes desirable for breeding (like the pinto gene that causes horses to have white spots on it coat) even after the horse has been gelded or passed away.

To see video clip visit our FaceBook page


COPY CAT'S TENTH BIRTHDAY!

The world's first cloned cat - Copy Cat or CC - will celebrate her tenth birthday in October! CC is living a happy, normal live in College Station, Texas with her mate Smokey and their kittens! CC has helped dispel animal cloning myths such as clones live shortened lives and are unable to reproduces. More images and accounts of CC are in the following link:

Click Here


DOG CLONING UPDATE

Melvin Dupont’s clones—Ken and Henry—are nearly 20 months old. Their owners Dr. Phillip and Paula Dupont continue to marvel at how similar the clones are to a young Melvin and to one another. Melvin on the other hand seems to be losing interest in his clones—possibly because he can’t keep pace with them!

Enjoy the another video of Melvin, Ken and Henry at the PerPETuate website: Click Here


FRADULENT DOG AND CAT CLONING COMPANIES

Beware of any pet DNA company that claims it can store your pet’s DNA for future cloning opportunities for less than a several hundred dollars. Cats, dogs and other animals can be cloned ONLY with viable undifferentiated DNA or cells. The biotechnology processes required to produce and store DNA or cells for cloning dogs and cats are complex and costly.

Cat and dog owners who are interested in storing non-viable DNA but not cloning their pets should contact legitimate genetic laboratories like the Veterinary Genetics Laboratory at the University of California in Davis, California. www.vgl.ucdavis.edu/contacts.html


SENIOR CATS

Cats like people and other pets are living longer. The renowned Cornell Feline Health Center at Cornell University in Ithaca, New York found that the percentage of cats over six years of age has nearly doubled in just over a decade and this trend is expected to continue.

You can read the Cornell Feline Health Center’s study and recommendations for Senior Cats at the following link:

http://www.vet.cornell.edu/FHC/health_resources/SeniorCats.cfm


2014 WORLD EQUESTRIAN GAMES

The World Equestrian Games kicks off today in Normandy. More than 70 nations, 1,000 athletes and as many horses will compete in internationally recognized styles of riding.

Last year the Federation Equestre International, the governing body of international equestrian sports, agreed to recognize equine clones and allow them to compete as equals in the World Equestrian Games. This landmark decision shows that cloning is being progressively recognized around the world. It will also help determine whether clones of previous champions can be as or more successful than their champion donors.

Tune in to www.FEITV.org now through September 2nd to keep up with these exciting equine athletes. #WEG2014


PET CLONING

Cloning should be viewed as a new procedure for breeding animals. Traditionally cats, dogs and horses have been bred naturally; selectively chosen then bred; bred by artificial insemination (AI) or reproduced by embryo transfer (ET). Selective, AI and ET breeding enable owners to produce genetically improved kittens, puppies or foals. Cloning is ultimate breeding procedure as it enable 99% of the desired genetic traits to be reproduced in the cloned cat, dog or horse.

For a more pet cloning explanations and illustrations please refer to the cloning section under Frequently Asked questions (FAQ) http://perpetuate.net/faq.html


PERPETUATE IS BACK!

Since our last posting PerPETuate revised its website; we preserved DNA from many new pets including our first Argentinian dog-Conan;
our genetic services for horses were enhanced; and PerPETuate continued and continues to improve our genetic preservation services and the development feline and canine cloning.

You are encouraged to check out PerPETuate’s new website at www.perpetuate.net Please leave your comments and recommendations by clicking ‘contact us’ and leave a message.


PERPETUATE, Inc. welcomes Pinto World Championship Horse Show visitors to the exciting 2014 show and to our website!

We encourage you to visit PERPETUATE's representative, April Culbertson of Black Magic Saddlebreds at the Exchange barn (X) aisle 10 stalls 1-3 to discuss PERPETUATE's unique biotech services.


GENES AND GENOTYPES

The Veterinarian Genetics Laboratory (VGL) at the University of California-Davis is internationally recognized as a pioneer and expert in DNA-based animal testing. For a decade VGL has provided PERPETUATE’s animal owners with genotypes to identify their pets, horses, cell lines and clones.

With each new VGL genetic discovery some genes that PERPETUATE has banked increase in value. PERPETUATE owners look forward to the day when VGL will be able to identify specific breeds using their genotypes.

Animal identification is a relatively junior genetic test when compared to VGL’s complex forensic services, parentage verification, genetic disease screening and diagnostic testing for a wide spectrum of domestic species, primates and wildlife. For example VGL recently announced a DNA test for Beagle Primary Open Angle Glaucoma.

To better appreciate VGL’s services check out their canine service at this link


NATIONAL PET CANCER AWARENESS MONTH

We here at PERPETUATE wanted to take a minute to remind everyone that May is National Pet Cancer Awareness Month. Cancer can be devastating to pets and pet owners alike so do your part and get informed on what the warning signs are to keep your exceptional pets healthy, happy and feeling their best!

What many people do not know is genotypes, a map of an animals genetic makeup, much like the genotypes we provide through our services on PERPETUATE.net are used in cancer research to identify and aid in the development of cures for various types of cancer. Genotypes were particularly useful in confirming that the canine cancer CTVT was in fact an 11,000 year old STD. To find out more about this cancer causing STD and how much scientists were able to uncover about this cancer through the use of genotypes click here


CALIFORNIA CHROME WINS THE KENTUCKY DERBY!

California Chrome, a Throughbred racehorse bred by a pair of relative unknowns and trained by the oldest trainer to have ever won the Kentucky Derby Art Sherman, has officially captured the hearts of America along with the first jewel of The Triple Crown. California Chrome’s owners, Steve Coburn and Perry Martin, are a pair of self proclaimed “dumbasses” that saw something incredible in the bloodlines of a mare that they bought for a meer $8,000 that many others had overlooked. That mare, Love That Chase whom had won only 3 races out of 22 starts, would go on to produce California Chrome and set into motion the Cinderella story that has unfolded. California Chrome’s story serves as a wake up call to all breeders across species lines that when it comes to breeding it’s the genes of the animal combined with the training of that animal that produces a champion, not the titles won by others present in it’s lineage. Here at PERPETUATE we strive to educate owners and breeders alike of the importance of genetic preservation but it is rare that we have an opportunity to address it in a way that so many people can relate. Had California Chrome’s owners not have bought Love That Chase and placed faith in breeding her her line may have ended with her and we the public would not have had the opportunity to witness the greatness that is California Chrome.

So how does genetic preservation and cloning play into Throughbred breeding? The Jockey Club, the registry that governs over breeding of Throughbred race horses, has taken a stance to not allow “unnatural” bred horses to be registered, meaning no horses concieved through practices like artificial insemination, cloning, etc. can be registered, in an attempt to follow tradition and maintain the gambling spirit of the sport of horse racing. What many people fail to realize, however, is that the Jockey Club is not the only Throughbred registry in North America. As with many breeds of horses today Throughbreed breeders realized that not only are they producing horses that are exceptionally fast but also incredible athletes that have potential to excel at many other internationally recognized equestrian sports. In 1994 the Performance Horse Registry was founded to document the acomplishments and lineage of Throughbreds outside of the track and have since opened up their services to provide full registration of horses from all breed backgrounds regardless of breeding practices. Since then Throughbreds have flourished in international sport horse disciplines including Dressage, Jumping, and Eventing. Last year the FEI, the governing body over international equestrian competitions, ruled that clones were able to compete as equals believing, much like the owners of California Chrome, that it’s not only good genes that make an exceptional horse but the combination of these genes and exceptional training that produce champions.

For those interested in following California Chrome’s quest for The Triple Crown the second race, the Preakness, is set for May 17th in Baltimore and incase you missed it the Kentucky Derby can be viewed here


140TH KENTUCKY DERBY

The most exciting two minutes in sports is less than an hour away and whether your a fan of horse racing or not there’s no doubt about it these horses are truly exceptional. Here at PERPETUATE we love to share with you all of the exceptional pets that we have had the fortune of working with over the years and are please to include horses as part of the PERPETUATE family. For those of you who are tuning into today’s race we would love to hear from you on which horse is truly going to stand out amongst the rest as the most exceptional in today’s run for the roses. If you havent placed your bet yet check out the lineup of horses here

Check back tomorrow and we’ll bring you an update on the winner and how thoroughbred racing impacts equine cloning.


NYC’S CAT CAFE SHOWS PEOPLE’S LOVE FOR CATS!

Cat lover’s rejoice! America’s first cat cafe opened in New York city last week and the outpouring of support from feline fans proves just how much people love their cats! Here at PERPETUATE we love each and every one of the exception pet’s that have joined the PERPETUATE family and we are puurrfectly pleased to see that people everywhere share that love for our feline friends and are incorporating that love in new and exciting ways. Click here to check out NYC’s cat cafe and see all the friendly felines that got to get in on the fun!


Penny PERPETUATE here today with my best friend Zsa Zsa!

Spring has sprung and with everything now fully in bloom we want to make sure you and your exception pets stay safe this season. While there is only a small percentage of plants that are toxic to pets it's always a good idea to stay informed on which ones to watch out for! Check out this link to review top ten most poisonous plants to your pet:


GENETIC PRESERVATION OF HORSES

Here at PERPETUATE we offer genetic preservation services for not only dogs and cats but a variety of animals. This week we would like to answer a few frequently asked questions about genetic preservation of horses.

How do horse owners start the genetic preservation process?

1. Order a cell collection kit from PERPETUATE.net
2. Have your veterinarian use the kit to collect and transport skin tissue samples.
3. Ship your tissue samples overnight to PERPETUATE's laboratory in Worchester, Massachusetts.
4. PERPETUATE processes skin tissue into a viable cell line.
5. PERPETUATE cryopreserves and stores your cell line.

How does my veterinarian collect tissue samples?

Included in your genetic preservation kit will be three collection vials and skin biopsy punches. Your veterinarian will select a biopsy site (commonly done under the mane area), apply a local anesthetic, shave and sterilize the area, then remove three small skin biopsies which are then placed in the vials to be shipped.

What do I do in the event my horse has recently died?

Call PERPETUATE immediately at (808) 989-2028 for emergency directions.


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To enter your special pet or horse in our program, call 808-989-2028
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