Pet and Horse Genetic Preservation
What's New and What's In the News
Gwen's clones are six months old! Their owners are amazed
by the behavioral and physical similarities between Gwen and
Physically they sleep in the same position and location as
did Gwen; their groomer noted similarities amongst their skulls
and they all look alike. Behaviorally the clones are playful
and loveable like Gwen and they share food preferences.
Most important the clones help fill the void created with
the passing of Gwen on May 15, 2017.
KEN AND HENRY CELEBRATE THEIR 5TH BIRTHDAY
Melvin was the best dog that Dr. Phillip and Paula Dupont
ever owned. When Melvin, a mixture of Catahoula and Doberman,
turned nine and began to age in 2012, the Duponts decided
to preserve his genetics.
Dr. Dupont biopsied two small pieces of Melvins skin
and sent them to PerPETuates laboratory. PerPETuate
successfully cultured and then cryopreserved more than three
million viable Melvin cells.
Phillip and Paula wanted to use these cells to clone Melvin
however they had two concerns. First, the cost to clone a
dog was $70,000! Second, only one biotech company was able
to clone dogs, the Sooam Biotech Research Foundation in South
The Duponts justified the cost by the fact that Melvin was
an exceptional dog. He greeted visitors to the Dupont veterinary
clinic with barking hellos and he had an uncanny ability to
understand English. Most important Melvin provided Paula and
Phillip with great pleasure. Besides, they reasoned $70,000
was less than the cost of a new Land Cruiser!
Next, the Duponts visited Sooam in South Korea. Being impressed
by the biotech staff, laboratory and facilities and by how
well the dogs were treated, they contracted Sooam to clone
Melvin. In mid-2012 PerPETuate shipped some of Melvins
cultured cells to South Korea. Sooam used these cells to produce
two clones of MelvinKen and Henry.
In spring of 2013, Ken and Henry joined Paula, Phillip and
Melvin in Louisiana. For the next two years, Melvin was a
role model for his clones. When Melvin did pass away having
two identical replicas helped ease the couples grief.
The Duponts say that Ken and Henry are more like Melvin every
day. Their personalities are identical, says Paula.
These pampered pooches are popular choices for cloning
Chihuahuas are finally getting the respect they deserve.
They may not be the most popular dog breed in America, but
they have something else to bark about.
The pint-sized pets lead the pack when it comes to the breeds
most commonly selected for cloning, said Ron Gillespie, founder
of PerPETuate, the aptly-titled Hawaii-based service that
preserves dogs genetic material so they can be cloned.
Though theres no hard data on dog cloning, Gillespie
has noticed some patterns in his 20 years in the canine copying
GUNNI, PerPETuates first 2018 clone, was released to
her owner Mrs. Monni Must on Jan 5th by ViaGen. Gunni and
Monni immediately bonded. Monni said Gunni has the same social
personality as her donorBilly Bean who is still living.
They both love people and are fearless. On the flight home
the whole airport took photos of Gunni and the Delta attendants
passed her around during the flight.
Gunni was well received by all except her donorBilly
Bean! Immediately Billy was envious of Gunni and would like
to have had her out of the house! After weeks of sensitive
management Billy and Gunni are sharing space and beginning
to form a close relationship.
Billy Bean and her clone Gunni are physically similar. Both
for example are smaller than most Black Labradors and have
very large paws. As Monni is a professional photographer we
should be able to track the physical similarities and differences
between Billy and Gunni.
Monnis assessment of her clone"Gunni is perfect!
LADY GUINEVERES CLONING TIMELINE:
May 15, 2017 Gwen died
May 18, 2017 Gwens tissue was collected
May 19, 2017 PerPETuate received and cultured Gwens
June 1, 2017 PerPETuate cryopreserved 4 million cultured cells
June 12, 2017 Transferred 1.6 million cells to ViaGen for
July 19, 2017 Created and transferred embryos to surrogate
Aug 8, 2017 ViaGen confirmed two Gwen pregnancies
Sept 22, 2017 Two Gwen clones were born
Now 10, 2017 Gwen clones arrived home in Philadelphia!
Glynn Greensmith interview of
PerPETutae's President on ABC
Australia Radio Sept 2017 Click Here to Listen.
Fibroblast cells are the primary cell type used to clone
dogs and cats. Skin and muscle, principal sources of fibroblast
cells, can be harvested relatively easy. Most important fibroblast
cells are undifferentiated. The following images are of large,
flat, elongated spindle-like fibroblast cells that PerPETuate
cultured from Nissa's skin biopsies.
Charlot's clones will soon celebrate their six-month birthdays.
Their owners said "they are all sweet, loveable and safely
made it through hurricane Irma." See postings from May
29 and June 5 for earlier images of these Papillion clones.
PET CLONING OBSERVATION #2
WILL MY CLONE'S HEALTH AND PHYSIQUE BE EQUAL TO THAT OF MY
Since 2008 approximately 25 dogs and cats have been cloned
from cells cultured by PerPETuate. While the number of clones
and observation period is limited, PerPETuate has found the
health, physical condition and size of clones is usually better
than their donors.
Clone health, physical condition and size should necessarily
be equal to or better than their donor. First clones are produced
from genes that are identical to the original pet's genes.
Second and most important, clones are treated throughout the
life with exceptional care. From birth to weaning they are
kept in hygienic conditions with their surrogate mothers;
they are observed regularly and handled by trained animal
caretakers and they receive comprehensive health care from
veterinarians. After weaning and for the rest of their lives
clone owners take exceptional care of their prized investments.
Seldom has the original pet been treated throughout their
life as well as their clone.
Doug and Michelle Shields' fluffy white Maltese Gwen lived
16.5 years before she died from a seizure. The Shields had
mulled the idea of preserving her genes in the past but it
wasn't until the fresh aftermath of her death on May 15, 2017
that they made the decision to start the cloning process.
The Shields reached out to PerPETuate, an animal genetic preservation
company, that successfully cultured and cryopreserved Gwen's
cells. These cells are now being used to clone Gwen.
"We're what you'd deem to be animal people. We have a parrot
and another dog we adopted," says Michelle. "But Gwen was
just an amazing, amazing, amazing dog. Just unbelievable.
She just had a personality. Everyone loved her. There was
no replacing her. So if I could get her back, or her personality
traits, I would do anything to do that." The Shields look
at the price tag as a worthy luxury. "Most of her friends
understand because they all loved Gwen too. We're just people
who really love our dog," Michelle says.
PERPETUATE is pleased to report that four clones of Charlot
will be delivered to their owners in Florida on June 1st.
The clones were born in April 2017. Charlot's cells were cryopreserved
by PerPETuate in January 2000. Charlot was a male Papillion.
PET CLONING OBSERVATIONS #1
PerPETuate cultures and cryopreserves genetics from exceptional
pets! Owners consistently state that their cat or dog was
the most incredible pet that they ever owned. Superlatives
like brilliant, extraordinary, gifted, incomparable, talented
and smart are repeatedly used to describe these unique animals.
PerPETuate believes the common denominator amongst these
remarkable animals is intelligence. Their extraordinary intelligence
enables them to learn easily and quickly, understand and establish
exceptional bonds with their owners.
After five years of observing a limited number of cloned
pets, PerPETuate is able and willing to make these preliminary
observations: - Cloned puppies and kittens from exceptional
donors appear to be more intelligent than normal progeny.
- Cloned puppies and kittens from exceptional donors are incredibly
happy, active and responsive to commands. PerPETuate expects
these observations to be scientifically confirmed in the future.
Cryopreservation is the final step in PerPETuate's cell culturing
process. Cryopreservation begins when cultured cells are transferred
from Petri dishes and to small vials of cryoprotectant medium.
After labeling, the vials are cooled slowly by a programmable
rate freezer to -112°F. The vials are then placed in liquid
nitrogen refrigerators at temperatures below -300°F. Cryopreserved
cells can be maintained in liquid nitrogen for decades.
PerPETuate stores our clients' cells in two separate storage
facilities. Pet owners can visit one of the facilities. The
second store's location is not disclosed for security reasons.
The following video explains cell cryopreservation in more
DOLLYS 20TH ANNIVERSARY
February 22nd. was the 20th anniversary of Dollys cloning
announcement. Dolly the sheep was born on July 5, 1996 in
Scotland. She was cloned at the Roslin Institute by Drs. Keith
Campbell and Ian Wilmuth.
Dolly had three mothers (one provided the egg, another the
DNA and a third carried the cloned embryo to term). She was
created using the technique of somatic cell nuclear transfer.
She was the first clone to be produced from an adult mammal
Dolly lived her entire life at the Roslin Institute in Edinburgh.
There she was bred with a Welsh Mountain ram and produced
six lambs in three pregnancies.
Dolly died from lung cancer on February 14, 2003. Any connection
between Dolly being a clone and her early death were dismissed.
First other sheep in Dollys flock died of the same disease
as she did. Second four other Dolly clones were produced after
Dolly all of which outlived her and are still living.
PerPETuate clients with stored non-viable tissue will be
interested in a new approach to cloning the woolly mammoth.
Harvard scientists are trying to rebuild the mammoth genome
by replacing some elephant genes with mammoth ones.
The new approach follows South Korean scientists' failure
to recover significant amounts of woolly mammoth DNA in Siberia.
Most of the uncovered mammoth tissue's DNA was destroyed after
being frozen for centuries. ... See
Woolly mammoth on verge of resurrection, scientists reveal
Scientist leading 'de-extinction' effort says Harvard team
could create hybrid mammoth-elephant embryo in two years
Pet Owner Question: "Our beloved old cat just started chemo
after being diagnosed with large cell intestinal lymphoma.
We would love to preserve his DNA but I am wondering if the
chemo and/or the cancer would affect the results? Have you
worked with others in this situation? Thanks!"
PerPETuate Answer: "We have successfully cultured cells from
hundreds of pets that were suffering with cancer and were
undergoing chemo or radiation treatment." Feb 4, 2017
CLONING TISSUE REQUIREMENTS
Pet owners frequently ask PerPETuate if fur, teeth or nails
can be used to clone their pets. The answer is 'no.' Even
when fur, teeth or nail tissue is viable, their cultured cells
cannot be used for cloning.
PerPETuate's practice is to use skin tissue from live and
muscle tissue from deceased animals to produce cell lines.
Adipose tissue is also acceptable. Blood, treated with heparin
to prevent coagulation, has in a very limited number of cases
produced viable pet cell lines. Cells cultured from these
four types of tissue can be used to clone pets.
PERPETUATE wishes our clients, friends and their pets HAPPY
PERPETUATE'S FIRST FELINE CLONES
PerPETuate's first feline clones are more than one year old.
Both clones were produced from a PerPETuate cell line at ViaGen's
cloning laboratory on September 27, 2015. The European owner,
who chooses to remain unidentified and not to publicize images
of his original cat, is completely satisfied with the clones.
Both clones are incredibly similar physically and behaviorally
to the original cat and to one another.
BOB SEMEL'S CLONES
Bob Semel, a spirited Deer Chihuahua, lost his life in September
2011. Clones of Bob, produced from cells cryopreserved by
PerPETuate, were born in January 2012. Bob's clones will celebrate
their 5th birthday next January.
Morpheus was the first Chausie that PerPETuate cell banked
in 2002. Chausie is a domestic breed of cat that was developed
by breeding domestic cats with a few species of nondomestic
jungle cats. Morpheus was tall, lean, with very long legs,
deep-chested, tall ears and striking eyes like other Chausies.
CLONED ANIMALS ARE HEALTHY AND SAFE
Twenty years after the birth of the worlds first cloneDolly
the Sheepextensive research has proven that cloned animals
are healthy, safe and age normally. These conclusions result
from analyzing thirteen clones of Dolly during the last fourteen
years. All of the clones including four, who are now eight
years old, are free of the diseases that caused Dollys
premature death when she was six years old.
Animal cloning is increasingly more reliable, better understood
and accepted by animal owners and the public. New cloning
applications like stem cell therapy, medical and behavioral
R&D, preserving endangered species, reviving extinct ones
and other unique prospects, bodes well for the future of animal
John Brannon, a dog trainer, has three clones of his top
police service dog. He trains dogs primarily to search for
drugs or explosives and to protect their handlers.
Brannon has successfully completed training of two of his
clones and placed them with police SWAT units. Training the
third cloneSpectoris going well.
Brannon says cloning seems to take the guess work out of
normal breeding procedures. He said even when you breed the
best female dogs with top male police dogs no more than 50%
can be trained to be police or military service dogs. See
following link for more information:
Dog and cat cloning are on verge of making significant progress
in near future. Pet cloning takes two formstherapeutic
Therapeutic cloning commonly known as stem cell therapy is
a burgeoning new industry focused on pet diseases and therapies.
Stem cells for example are employed increasingly to treat
osteoarthritis and bone, joint and ligament injuries for dogs
and stomatitis and other chronic cat diseases.
Reproductive cloning has made less progress than pet therapeutic
cloning. No more than twenty cats were cloned after 2003 when
the first cat was cloned. The number of dogs cloned since
2005 is estimated to be near 300 all of which were cloned
in South Korea. In past year improved cloning biotechnologies
and significant American investments in pet cloning are promising
news for pet owners.
Thanks for the great communications during the process, it
was an unexpected plus. There is a sense of security for the
family having completed the cell line. When I have discussed
with friends and colleagues none thought it 'odd' - or at
least said so to me. PR - April 2016
SHANNON DILLER CLONES
Media mogul Barry Diller, the chairman of IAC, and his wife,
designer Diane von Furstenberg have cloned their famous Jack
Russell terrier--Shannon. They are now the proud owners of
cloned pups, Deena and Evita.
Cloning dogs costs up to $100,000 and is done by a Korean
firm that implants DNA into a dog egg. A rep for Diller confirmed
SCIENTISTS IN IRAN CLONE RARE MOUFLON
A domestic sheep has given birth to a baby mouflon in a rare
successful example of interspecies cloning, according to scientists
in Iran. The wild Isfahan mouflon - or ovis orientalis isphahanica
- was cloned by researchers at Iran's Royan Institute, which
is dedicated to reproductive biomedicine and stem cell research,
using the domestic sheep as a surrogate mother. Poaching has
driven the Isfahan mouflon close to extinction in Iran. The
cloned mouflon, which looks similar to a deer, has been named
Maral, a Persian name for a reindeer and for new babies, which
means svelte. For more information click
SCOTTISH SCIENTISTS ANNOUNCE PLANS TO CLONE ENDANGERED GIANT
Currently, there are only about 2,000 giant pandas left in
the world and in an attempt to save giant pandas from extinction,
scientists have announced plans to clone Tian Tian and Yang
Guang who currently reside at Edinburgh Zoo in Scotland. The
same experts who cloned Dolly the sheep have already taken
tissue samples from the giant pandas and produced viable cell
lines to genetically preserve them for later cloning use.
This cloning project is the first step in making genetic copies
of the endangered animals following a failed attempt to artificially
inseminate Tian Tian in March. For more information click
KOREAN CLONES TO ENTER SERVICE AS RESCUE DOGS
Dasol and Nara, two dogs cloned by dog cloning pioneer Hwang
Woo-suk and his Sooam biotech team, have been accepted into
Korea's rescue dog program becoming what are assumed to be
the world's first clones to enter service as rescue dogs.
The National 119 Rescue Service of the Ministry of Public
Safety and Security announced that the two clones donated
by Hwang, Dasol and Nara, have passed the official qualification
tests for rescue dogs. Both dogs were cloned from donors whom
had previously been enlisted as service dogs and are showing
great promise to follow in the same footsteps. Read more here:
EXTINCT PREHISTORIC CAVE LION UNDER CONSIDERATION FOR CLONING
BY RUSSIAN SCIENTISTS
In November Russian scientists discovered the best preserved
remains to date of two baby cave lions whom likely died out
12,000 years ago around the same time as the Mammoths. "The
remains are unique and know no equals across the world," the
local Academy of Sciences' statement reads. "Since the
cubs have well-preserved soft tissues, we, the scientists,
tend to believe that they can be cloned. But we can speak
about the results of this project in two or three years."
Scientists from Japan, South Korea and the United States are
joining in the study. Read more: http://ow.ly/V5y3L
FIRST CLONED CAMEL GIVES BIRTH IN DUBAI
The first cloned camel gave birth to a healthy happy female
calf on Nov. 2nd at the Reproductive Biotechnology Center
in Dubai. Several more cloned camels are due to give birth
next year. For more information visit http://ow.ly/V5xYv
Pets are our friends, our confidants and our constant companions.
Whether they're devoting their time to their loving families
or dedicating their lives to service work our furry companions
are constantly giving back. This year join us in giving thanks
for all the wonderful pets that have touched our lives and
give back to them by giving them the gift of genetic preservation.
PerPETuate preserves DNA by culturing and cryopreserving viable
cells from exceptional pets and horses. Cryopreserved cells
can be used to clone cats, dogs and horses, perpetually preserve
their bloodlines and for medical diagnosis and treatment.
Pet hospices provide comfort-oriented care to companion animals
as they approach the end of life. The professional pet hospice
movement began in the 1990s. Since then the number of pet
owners who use and number of veterinarians who offer hospice
services have and are increasing. The International Association
of Animal Hospice and Palliative Care was formed a few years
ago to develop guidelines and to promote research and scholarly
discussion animal hospice practices and issues.
Bob Semel's clones are now fully grown and nearly four years
PerPETuate clients and friends will remember Bob Semel and
his three clones. The clones were born in South Korea in January
2012 following Bob's tragic death in August 2011. The three
clones-Bob, Jim and Tom-were some of the first dogs to be
produced from a PerPETuate cell line.
From the time the clones reached their Los Angeles home in
March 2012, Dr. George Semel their owner continued to be amazed
with how similar the clones were to Bob and to one another
in terms of temperament, personality and physically. One of
the three clones-Tom-was lost in a traffic accident in when
he was a year old. The other two clones-Jim and Bob-are now
fully grown, healthy and full of life. See video of clones
in post below.
FIRST ANNUAL DOG FILM FESTIVAL SET FOR THIS WEEK
The First annual dog film festival will take place on October
3, 2015 from 11 a.m. - 11 p.m. at the prestigious Peter Norton
Symphony Space, a 765-seat theater in New York City. The festival
has a full line up set to delight from doggy swag bags to
"dogumentaries" it's poised to be a barking good
DNA profiling was developed and first used in 1985 to identify
individuals by their DNA traits. DNA profiles are comprised
of a limited number of specific DNA genes that are unique
to an individual like fingerprints. Today DNA testing is used
extensively to identify individual persons and for forensic
In the 1990s the International Society of Animal Genetics
began developing standardized DNA profiles for pets and livestock.
DNA profiles are known alternatively as DNA signatures or
fingerprints, genetic-marker reports, testing and genotypes.
PerPETuate maintains that DNA profiles present a method to
certify scientifically animal clones. The donor pet's DNA
signature must be identical to the clone's DNA signature or
it is not a clone! Since 2000 the University of California-Davis
Veterinary Genetic Laboratory has provided PerPETuate clients
with DNA signatures of their pets.
DNA profiles use is limited to the identification of the
individual animal and its parents, cells and clones. Other
types of DNA biotechnology such as sequencing techniques can
be used to determine the breed, color, disease, lineage and
De-extinction is the process of creating an organism that
is or resembles a member of an extinct species. De-extinction
efforts have not yet succeeded in producing viable offspring
of a previously extinct species, the process however has been
applied successfully to endangered species such as the gaur.
PerPETuate has cryopreserved non-viable cells from many pets
that could not be cultured often due to freezing. Our clients
are anxiously following the advancement of de-extinction research
and development with the hope that will enable them to clone
their pets in the future.
New York's J. F. Kennedy Airport can look forward to a new
luxury terminal - The ARK- that will handle the more than
70,000 animals flying in and out the USA every year. The ARK
will be the world's first air terminal for pets and other
animals. Set to open next year the $48 million, 178,000-square-foot
shelter and quarantine facility will be able to accept all
species and large numbers of pets and animals.
The ARK will offer a 20,000-square-foot "resort" run with
a bone-shaped splashing pool for dogs and cats will have their
own trees to climb. All animals will have access to a 24-hour
clinic operated by Cornell University's veterinary college
Dogs are the most difficult of all mammals to clone. Problems
begin with fact that the canine reproductive physiology is
significantly different from other species. Many procedures
used in livestock cloning cannot be applied to dogs.
The limited supply of canine oocytes or immature eggs is
another important challenge. Large numbers of oocytes are
needed to clone dogs. Canine females tend to ovulate randomly
and only twice a year. In addition they produce fewer eggs
per ovulation than do other species. And mating does not trigger
Another major challenge is that after an oocyte matures it
is viable for only a few hours versus several days for other
species. The entire canine cloning process from egg collection,
evaluation and synchronization to somatic cell nuclear transfer
and finally to transferring the cloned embryo to a surrogate
mother must be completed within no more than ten hours!
GENETIC ENGINEERING: THE INNATE POTATO
The Innate Potato is a GMO creation that was recently approved
for cultivation in the US. Genes from a wild Mexican variety
of potato were added to the popular species of Russet Burbank
potatoes resulting in the Innate Potato.
The Innate Potato is an example of how genetic engineering
is positively benefiting mankind as it:
Reduces the amount of acrylamide produced by potatoes
by as much as 70%. Acrylamide is a known carcinogen at high
Reduces some of the $300 million annual potato waste
caused by bruising. Potato bruising results from enzyme released
during harvest and transport. Enzyme release is controlled
in the Innate Potato.
Improves starch quality and potato color.
Requires no additional pesticides or chemicals.
Does not sequence cross species.
Is not linked to Monsanto.
Skin from living pets is the best source of tissue for genetic
culturing and preservation. PerPETuate recommends that a biopsy
punch with its circular blade be used to collect two 3 to
4 mm full-thickness skin samples (each approximately size
of pencil eraser). The procedure begins by administering a
local or general anesthesia and then by shaving and sterilizing
the biopsy site. Skin punch biopsies usually take less than
30 minutes to complete.
Biopsy Site Selection: Skin samples should not be taken from
areas where bacteria tend to concentrate such as feet, rump
and mouth. Possible infected skin should be avoided. The scruff
of the neck is an advantageous site as it is easily assailable,
clean and cannot be licked by the pet after the biopsy.
Scientists have completed mapping the woolly mammoth genome.
This genetic road map will be of considerable use in efforts
underway to clone the woolly mammoth. It also underscores
the value and potential of animal genetic preservation.
Scientists Map Mammoth Genomes, Which Could Help Bring Beasts
Back To Life
Scientists may be one step closer to bringing the woolly
mammoth back to life. In a new study, an international team
of researchers has sequenced the genomes of ... Click
here for more
BIOPSY FACTS AND RECOMMENDATIONS
Genetic preservation requires small portions of viable
tissue from your pet.
Skin is the preferred type and source of tissue from
Muscle is the preferred type and source of tissue from
Tissue must be harvested in a germ-free environment.
Local or general anesthesia is necessary for skin sample
Combining skin collection with teeth cleaning or other
operation is recommended.
Tissue needs to be collected as soon as possible from
It is recommended that tissue be collected immediately
after a pet is euthanized.
Drugs used to euthanize animals do not affect cell
Deceased animals must be kept at refrigerated temperatures
until tissue is harvested.
Tissue usually loses its viability within five days
Call PerPETuate for more information at 808-989-202.
ENDANGERED AND EXTINCT SPECIES
PerPETuate's primary goal is to preserve genetics from exceptional
pets and horses enabling these genes to be used for cloning
or genetic improvement. Additionally PerPETuate encourages
the use of genetic preservation and cloning for endangered
breeds and species. For example in 2001 PerPETuate was associated
with Advanced Cell Technology when they cloned a gaur--an
endangered Asian ox.
Since PerPETuate's founding in 1998 the use of genetic preservation,
even cloning endangered animal and plant species has achieved
worldwide acceptance and practice. Today scientists are pursuing
the much more daunting task of reviving extinct species through
March 1st marks the 43rd annual celebration of National
Pig a day, a holiday created to accord the pig its rightful,
though generally unrecognized, place as one of man's most
intellectual and domesticated animals. This month also serves
as the 15th anniversery of the announcement of the very first
pig clones produced by a team of PPL Theraputic scientists
in Blacksburg, VA and PerPETuates very own lab manager,
Dr. Ray Page, was part of the team.
Happy Birthday Charles Darwin!
Charles Darwin revolutionized the world with his theories
on evolution and inherited traits passed on from parents to
offspring in the early 1800's. Now, more than two centuries
later, the ideas set forth by the great grandfather of genetics
has not only shaped our views on breeding plants and animals
but opened our eyes and minds to the infinite possibilities
of genetic research. February 12th marked the 206th anniversary
of the birth of Charles Darwin and a summary of his impact
on genetics through the years can be found
Since the dog genome was sequenced ten years ago geneticists
have identified hundreds of canine diseases and physical trait
genes. By comparison only a limited number of such genes have
been identified in cats.
In 2007 the first cat genome was sequenced. Errors and gaps
however stalled efforts to map genes. A complete, high-resolution
feline genome was published in 2014. One if its first discoveries
was that cats after 9,000 years of domestication are quite
similar to their original feral counterparts!
Geneticist Leslie Lyons of the University of Missouri has
launched a feline genetics research project called 99
Lives Initiative to improve the quality of data and
identify genetic variations behind specific feline diseases.
Humans stand to benefit from 99 Lives as cats
and humans share similar diseases such as type 2 diabetes,
asthma, retinal atrophy and HIV.
PerPETuate recently preserved genetics from Karma, a beautiful
female African Lion. Following Karmas untimely death
The Black Jaguar-White Tiger Foundation undertook extraordinary
efforts to hand carry and deliver Karmas blood samples
from Mexico City to PerPETuates laboratory in Worcester,
The Black Jaguar-White Tiger Foundation rescues cruelly
treated Big Cats from circuses, zoos and illegal breeders.
In 2013 the Foundations founder rescued a newly born
Black Panther cub about to be euthanized after her mother
died during birth. Since then the Black Jaguar-White Tiger
Foundation has rescued more than 30 lions, tigers and jaguars.
You can learn more and support The Black Jaguar-White Tiger
Foundation by visiting their website: www.blackjaguarwhitetiger.org
TIBETAN MASTIFF CLONES
Three Tibetan Mastiff clones were produced in China. Tibetan
Mastiffs are one of the oldest, rarest and most expensive
canine breeds. Tibetan herders have used them as shepherd
dogs since 1100 BC. They are solemn and fierce simultaneously
kind and loyal. Their immense double coat protects them from
sever Himalayan cold weather. They can be black, brown and
blue and may have tan or gold markings.
Visit our Facebook page and enjoy a compilation of our fabulous
pets from 2014 as they wish you a very Happy 2015!. See
Penny PerPETuate here along with my trusty reindog sledding
by to wish you and yours Happy Holidays!
Pope Francis recently consoled a small child whose dog had
died by assuring him that one day, we will see our animals
again and that paradise is open to all of Gods
creatures according to widely cited Italian media reports.
So don't forget to spread the good cheer! Genetic preservation
from PerPETuate, Inc. Is the only gift this holiday season
that gives pet owners the piece of mind that their exceptional
pet's DNA is preserved indefinitely. Genetic preservation
not only offers pet owners a head start on cloning but also
access to their exceptional pet's genes that can be used to
check for genetic diseases and hereditary traits as tests
become available. Order your $100 tissue collection kit today
After 9,000-odd years of living alongside humans, the house
cat is only semi-domesticated according to recent DNA studies
by scientists at Washington University in St. Louis. Domestic
cats still have many of the same traits as their wild cousins.
For example the fact that house cats unlike dogs retain their
ability to hunt and survive effortlessly in the wild underscores
how little impact humans have had on them.
The genomic study on the other hand concluded that the humans
have had some influence on cats. For example stem cell changes
have resulted in more docile felines and humans have selectively
developed cat breeds for preferred coat colors, patterns and
Penny PerPETuate here to wish everyone a magical Happy
Penny and the entire PerPETuate family hopes you and your
exceptional pets have a cackling good time today!
NATIONAL CAT DAY
National Cat Day is October 29. PerPETuate loves to celebrate
our feline friends! What better way to celebrate your exceptional
kitty than to preserve his or her genes for years to come?
PerPETuate Inc. the worlds most experienced genetic
preservation company, has been genetically preserving exceptional
cats since 1998. For more info on how to take the first steps
towards genetically preserving your pet visit www.perpetuate.net
REPTILE GENETIC PRESERVATION
As National Reptile Awareness Day nears, PerPETuate expects
more questions regarding whether it is possible to clone turtles,
snakes, lizards or other species of reptiles. We believe it
is possible to clone reptiles but know of no successful cloning
attempt to date.
PerPETuate can develop reptile cell lines and preserve their
genetics for future cloning. Preserving reptile cells involves
a different procedure than used in preserving dog and cat
genetics. For more information contact PerPETuate at 808-989-2028.
PRESERVING CHAMPION GENES FOR FUTURE GENERATIONS
Genetic preservation offers horse owners a unique new opportunity
to perpetuate the bloodlines of past champions for future
generations. Highly competitive horses are at a greater risk
of illness, injury and accidental death due to the rigors
of training, traveling to competitions, and the increased
risk of exposure to disease at these big events. By genetically
preserving these horses owners are insured the bloodlines
of these horses remain safe and give owners the opportunity
to have their "once in a lifetime horse" twice through
cloning. The benefits of genetic preservation aren't just
limited to cloning possibilities, they also allow stallion
owners the opportunity to test for specific genes desirable
for breeding (like the pinto gene that causes horses to have
white spots on it coat) even after the horse has been gelded
or passed away.
The world's first cloned cat - Copy Cat or CC - will celebrate
her tenth birthday in October! CC is living a happy, normal
live in College Station, Texas with her mate Smokey and their
kittens! CC has helped dispel animal cloning myths such as
clones live shortened lives and are unable to reproduces.
More images and accounts of CC are in the following link:
Melvin Duponts clonesKen and Henryare nearly
20 months old. Their owners Dr. Phillip and Paula Dupont continue
to marvel at how similar the clones are to a young Melvin
and to one another. Melvin on the other hand seems to be losing
interest in his clonespossibly because he cant
keep pace with them!
Enjoy the another video of Melvin, Ken and Henry at the PerPETuate
website: Click Here
FRADULENT DOG AND CAT CLONING COMPANIES
Beware of any pet DNA company that claims it can store your
pets DNA for future cloning opportunities for less than
a several hundred dollars. Cats, dogs and other animals can
be cloned ONLY with viable undifferentiated DNA or cells.
The biotechnology processes required to produce and store
DNA or cells for cloning dogs and cats are complex and costly.
Cat and dog owners who are interested in storing non-viable
DNA but not cloning their pets should contact legitimate genetic
laboratories like the Veterinary Genetics Laboratory at the
University of California in Davis, California. www.vgl.ucdavis.edu/contacts.html
Cats like people and other pets are living longer. The renowned
Cornell Feline Health Center at Cornell University in Ithaca,
New York found that the percentage of cats over six years
of age has nearly doubled in just over a decade and this trend
is expected to continue.
You can read the Cornell Feline Health Centers study
and recommendations for Senior Cats at the following link:
The World Equestrian Games kicks off today in Normandy. More
than 70 nations, 1,000 athletes and as many horses will compete
in internationally recognized styles of riding.
Last year the Federation Equestre International, the governing
body of international equestrian sports, agreed to recognize
equine clones and allow them to compete as equals in the World
Equestrian Games. This landmark decision shows that cloning
is being progressively recognized around the world. It will
also help determine whether clones of previous champions can
be as or more successful than their champion donors.
Tune in to www.FEITV.org
now through September 2nd to keep up with these exciting equine
Cloning should be viewed as a new procedure for breeding
animals. Traditionally cats, dogs and horses have been bred
naturally; selectively chosen then bred; bred by artificial
insemination (AI) or reproduced by embryo transfer (ET). Selective,
AI and ET breeding enable owners to produce genetically improved
kittens, puppies or foals. Cloning is ultimate breeding procedure
as it enable 99% of the desired genetic traits to be reproduced
in the cloned cat, dog or horse.
For a more pet cloning explanations and illustrations please
refer to the cloning section under Frequently Asked questions
PERPETUATE IS BACK!
Since our last posting PerPETuate revised its website; we
preserved DNA from many new pets including our first Argentinian
our genetic services for horses were enhanced; and PerPETuate
continued and continues to improve our genetic preservation
services and the development feline and canine cloning.
You are encouraged to check out PerPETuates new website
at www.perpetuate.net Please leave
your comments and recommendations by clicking contact
us and leave a message.
Inc. welcomes Pinto World Championship Horse Show visitors
to the exciting 2014 show and to our website!
We encourage you to visit PERPETUATE's
representative, April Culbertson of Black Magic Saddlebreds
at the Exchange barn (X) aisle 10 stalls 1-3 to discuss PERPETUATE's
unique biotech services.
GENES AND GENOTYPES
The Veterinarian Genetics Laboratory (VGL) at the University
of California-Davis is internationally recognized as a pioneer
and expert in DNA-based animal testing. For a decade VGL has
animal owners with genotypes to identify their pets, horses,
cell lines and clones.
With each new VGL genetic discovery some genes that PERPETUATE
has banked increase in value. PERPETUATE
owners look forward to the day when VGL will be able to identify
specific breeds using their genotypes.
Animal identification is a relatively junior genetic test
when compared to VGLs complex forensic services, parentage
verification, genetic disease screening and diagnostic testing
for a wide spectrum of domestic species, primates and wildlife.
For example VGL recently announced a DNA test for Beagle Primary
Open Angle Glaucoma.
To better appreciate VGLs services check out their
canine service at this
NATIONAL PET CANCER AWARENESS MONTH
We here at PERPETUATE
wanted to take a minute to remind everyone that May is National
Pet Cancer Awareness Month. Cancer can be devastating to pets
and pet owners alike so do your part and get informed on what
the warning signs are to keep your exceptional pets healthy,
happy and feeling their best!
What many people do not know is genotypes, a map of an animals
genetic makeup, much like the genotypes we provide through
our services on PERPETUATE.net
are used in cancer research to identify and aid in the development
of cures for various types of cancer. Genotypes were particularly
useful in confirming that the canine cancer CTVT was in fact
an 11,000 year old STD. To find out more about this cancer
causing STD and how much scientists were able to uncover about
this cancer through the use of genotypes click
CALIFORNIA CHROME WINS THE KENTUCKY DERBY!
California Chrome, a Throughbred racehorse bred by a pair
of relative unknowns and trained by the oldest trainer to
have ever won the Kentucky Derby Art Sherman, has officially
captured the hearts of America along with the first jewel
of The Triple Crown. California Chromes owners, Steve
Coburn and Perry Martin, are a pair of self proclaimed dumbasses
that saw something incredible in the bloodlines of a mare
that they bought for a meer $8,000 that many others had overlooked.
That mare, Love That Chase whom had won only 3 races out of
22 starts, would go on to produce California Chrome and set
into motion the Cinderella story that has unfolded. California
Chromes story serves as a wake up call to all breeders
across species lines that when it comes to breeding its
the genes of the animal combined with the training of that
animal that produces a champion, not the titles won by others
present in its lineage. Here at PERPETUATE
we strive to educate owners and breeders alike of the importance
of genetic preservation but it is rare that we have an opportunity
to address it in a way that so many people can relate. Had
California Chromes owners not have bought Love That
Chase and placed faith in breeding her her line may have ended
with her and we the public would not have had the opportunity
to witness the greatness that is California Chrome.
So how does genetic preservation and cloning play into Throughbred
breeding? The Jockey Club, the registry that governs over
breeding of Throughbred race horses, has taken a stance to
not allow unnatural bred horses to be registered,
meaning no horses concieved through practices like artificial
insemination, cloning, etc. can be registered, in an attempt
to follow tradition and maintain the gambling spirit of the
sport of horse racing. What many people fail to realize, however,
is that the Jockey Club is not the only Throughbred registry
in North America. As with many breeds of horses today Throughbreed
breeders realized that not only are they producing horses
that are exceptionally fast but also incredible athletes that
have potential to excel at many other internationally recognized
equestrian sports. In 1994 the Performance Horse Registry
was founded to document the acomplishments and lineage of
Throughbreds outside of the track and have since opened up
their services to provide full registration of horses from
all breed backgrounds regardless of breeding practices. Since
then Throughbreds have flourished in international sport horse
disciplines including Dressage, Jumping, and Eventing. Last
year the FEI, the governing body over international equestrian
competitions, ruled that clones were able to compete as equals
believing, much like the owners of California Chrome, that
its not only good genes that make an exceptional horse
but the combination of these genes and exceptional training
that produce champions.
For those interested in following California Chromes
quest for The Triple Crown the second race, the Preakness,
is set for May 17th in Baltimore and incase you missed it
the Kentucky Derby can be viewed
140TH KENTUCKY DERBY
The most exciting two minutes in sports is less than an hour
away and whether your a fan of horse racing or not theres
no doubt about it these horses are truly exceptional. Here
we love to share with you all of the exceptional pets that
we have had the fortune of working with over the years and
are please to include horses as part of the PERPETUATE
family. For those of you who are tuning into todays
race we would love to hear from you on which horse is truly
going to stand out amongst the rest as the most exceptional
in todays run for the roses. If you havent placed your
bet yet check out the lineup of horses here
Check back tomorrow and well bring you an update on
the winner and how thoroughbred racing impacts equine cloning.
NYCS CAT CAFE SHOWS PEOPLES LOVE FOR CATS!
Cat lovers rejoice! Americas first cat cafe opened
in New York city last week and the outpouring of support from
feline fans proves just how much people love their cats! Here
we love each and every one of the exception pets that
have joined the PERPETUATE
family and we are puurrfectly pleased to see that people everywhere
share that love for our feline friends and are incorporating
that love in new and exciting ways. Click here to check
out NYCs cat cafe and see all the friendly felines
that got to get in on the fun!
here today with my best friend Zsa Zsa!
Spring has sprung and with everything now fully in bloom
we want to make sure you and your exception pets stay safe
this season. While there is only a small percentage of plants
that are toxic to pets it's always a good idea to stay informed
on which ones to watch out for! Check
out this link to review top ten most poisonous plants
to your pet:
GENETIC PRESERVATION OF HORSES
Here at PERPETUATE
we offer genetic preservation services for not only dogs and
cats but a variety of animals. This week we would like to
answer a few frequently asked questions about genetic preservation
How do horse owners start the genetic preservation process?
1. Order a cell collection kit from PERPETUATE.net
2. Have your veterinarian use the kit to collect and transport
skin tissue samples.
3. Ship your tissue samples overnight to PERPETUATE's
laboratory in Worchester, Massachusetts.
processes skin tissue into a viable cell line.
cryopreserves and stores your cell line.
How does my veterinarian collect tissue samples?
Included in your genetic preservation kit will be three collection
vials and skin biopsy punches. Your veterinarian will select
a biopsy site (commonly done under the mane area), apply a
local anesthetic, shave and sterilize the area, then remove
three small skin biopsies which are then placed in the vials
to be shipped.
What do I do in the event my horse has recently died?
immediately at (808) 989-2028 for emergency directions.